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Coli by quantitatively overexpressing each component. Targeted proteomic assays and intermediate assays were used to determine the metabolic status of each mutant. Through targeted engineering, farnesene production could be increased predictably step by step, up to 1.1 g/L (2,000 fold) 96 h after induction at the shake-flask scale.
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Cornmeal was her main source of nutrition and occasionally meat of some kind as her family had the privilege to hunt and fish. Most of her early childhood was spent with her grandmother who was too old for slave labor.