Prokaryotes do not have introns (Except Archaebacteria). Therefore mRNA processing is not required. The primary mRNA transcript in eukaryotes undergoes processing and splicing to change into a functional mRNA. In prokaryotes splicing of mRNA transcript does not occur. Format: Abstract Send to See comment in PubMed Commons below. Anal Biochem. 1987 Dec;167(2 245-60. Baracchini E 1, Bremer H. Author information 1Biology Program, University of Texas, Dallas. Abstract A method has been developed to determine the synthesis rate and lifetime of bacterial mRNAs, either bulk mRNA. PMID : 2450483 Publication Types, MeSH Terms, Substances, Grant Support. Publication Types Research Support, U.S. Gov't, P.H.S. MeSH Terms Computer Simulation Escherichia coli/metabolism. Kinetics Nucleic Acid Hybridization Nucleic Acid Precursors/metabolism RNA, Bacterial/biosynthesis. The method uses hybridization of pulse-labeled RNA to specific probes followed by an evaluation based on a computer simulation of the labeling kinetics of different classes of RNA. The method was applied to the determination of bulk mRNA in Escherichia coli growing in glucose minimal medium: 60.
Three initiating factors found in prokaryotes. PIF-1, PIF-2, PIF-3 In eukaryotes small subunit of ribosome (40 S) gets dissociated with the initiator amino acyl tRNA (Met-tRNA Met) without the help of mRNA.
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The first amino acid methionine entering the ribosome is not formylated. The first amino acid methionine is formylated into N formyl methionine. The pre imitation complex formation is initiated by nine initiated factors.