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Messenger RNA - Wikipedia, the free encyclopedia

Initiation of Protein Synthesis in Bacteria

Prokaryotes do not have introns (Except Archaebacteria). Therefore mRNA processing is not required. The primary mRNA transcript in eukaryotes undergoes processing and splicing to change into a functional mRNA. In prokaryotes splicing of mRNA  transcript does not occur. Format: Abstract Send to See comment in PubMed Commons below. Anal Biochem. 1987 Dec;167(2 245-60. Baracchini E 1, Bremer H. Author information 1Biology Program, University of Texas, Dallas. Abstract A method has been developed to determine the synthesis rate and lifetime of bacterial mRNAs, either bulk mRNA. PMID : 2450483 Publication Types, MeSH Terms, Substances, Grant Support. Publication Types Research Support, U.S. Gov't, P.H.S. MeSH Terms Computer Simulation Escherichia coli/metabolism. Kinetics Nucleic Acid Hybridization Nucleic Acid Precursors/metabolism RNA, Bacterial/biosynthesis. The method uses hybridization of pulse-labeled RNA to specific probes followed by an evaluation based on a computer simulation of the labeling kinetics of different classes of RNA. The method was applied to the determination of bulk mRNA in Escherichia coli growing in glucose minimal medium: 60.

Three initiating factors found in prokaryotes. PIF-1, PIF-2, PIF-3 In eukaryotes small subunit of ribosome (40 S) gets dissociated with the initiator amino acyl tRNA (Met-tRNA Met) without the help of mRNA.

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The first amino acid methionine entering the ribosome is not formylated. The first amino acid methionine is formylated into N formyl methionine. The pre imitation complex formation is initiated by nine initiated factors.

Mrna biosynthesis bacteria
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